The annual "Ribbon of Life" stage spectacular is Golden Rainbow's single largest source of funding. Staged each June, "Ribbon of Life" is an incredible production show featuring hundreds of singers, dancers and other performers from the best shows in Las Vegas, staging original numbers created specifically for that year's "Ribbon of Life" show. Each performer donates his or her time and talent to the production. Easily the biggest show of the year, "Ribbon of Life" is a theatrical event for all ages that leaves audiences breathless, on their feet and cheering for more. This year marked the 23rd annual "Ribbon of Life", which was held June 28, 2009 at the Las Vegas Hilton at 1:00pm.

Last year’s “Ribbon of Life” production show raised more than $300,000 for Golden Rainbow to support people affected by HIV/AIDS in Clark County. The total was the largest ever raised by the organization from the show.